5-羥色胺

5-羥色胺又稱為血清素，簡寫為5-HT，是色氨酸代謝的中間產(chǎn)物，是一種研究充分的神經(jīng)遞質(zhì)，也可能作為一種外周激素。合成主要發(fā)生在胃腸道的腸嗜鉻細胞（ec細胞）和神經(jīng)元中。它高濃度存在于腸道的ec細胞、大腦的5-羥色胺能神經(jīng)元和血小板中。血清素主要降解為5-羥基吲哚乙酸（5-HIAA）或褪黑素，可通過尿液排出。在血液中，絕大多數(shù)的血清素存在于血小板中，并且很容易在血清中檢測到。血清和/或尿液中血清素水平的改變可能表明身體和心理功能障礙。在各種情況下，血清和/或尿液中的血清素平衡可能受損。例如，與未受影響的受試者相比，抑郁、焦慮甚至疼痛敏感性患者的血清素水平已經(jīng)下降。另一方面，在分泌血清素的神經(jīng)內(nèi)分泌腫瘤，也稱為類癌或肝細胞癌患者中，血清素水平升高。

產(chǎn)品資料

【產(chǎn)品名稱】

通用名稱：血清素快速檢測試劑盒（酶聯(lián)免疫法）

英文名稱：Serotonin FAST ELISA

 產(chǎn)品規(guī)格：96T

產(chǎn)品貨號：EIA-5061

測定意義

本試劑盒可用于定量檢測血清和尿液中的5-羥色胺（血清素），以評估其體內(nèi)穩(wěn)態(tài)。僅用于體外檢測。而尿液中血清素的檢測有助于神經(jīng)應(yīng)激癥的測定。近年來，隨著經(jīng)濟增長放緩帶來的一系列社會問題，部分人群因無法承受生活的重壓，抑郁風險明顯增加，青少年自殺和名人輕生的現(xiàn)象不時見諸于新聞。這應(yīng)當引起我們的重視。如何及時發(fā)現(xiàn)抑郁癥，并監(jiān)測診療措施有效性，減少悲劇的發(fā)生？這正是許多醫(yī)學研究者想要解決的問題，5-羥色胺（5-HT）、褪黑素等情緒激素的檢測對抑郁的研究有重要意義，科潤達生物將在本文介紹一款德國DRG原裝進口ELISA試劑盒：5-羥色胺試劑盒（5-HT），而在此之前，我們應(yīng)先對抑郁癥和抑郁情緒進行區(qū)別：

測定原理

此5-HT酶免試劑盒（EIA-5061）基于ELISA原理。在檢測中，血清素被定量�；�。隨后使用的競爭性ELISA試劑盒中，微孔板上包被了抗原。�；�的標準品、質(zhì)控品及樣本和固相結(jié)合的分析物競爭固定數(shù)量的抗血清結(jié)合位點。在該系統(tǒng)達到平衡后，游離抗原和游離抗原-抗血清復合物通過洗滌被除去。固相結(jié)合的抗體通過抗兔IgG-過氧化物酶共軛物使用TMB為底物來檢測。測量450nm處的吸光度。未知樣本的定量通過與已知標準品濃度構(gòu)建的參考曲線進行比較來實現(xiàn)。

標準配置

1）微孔板：          12x8孔

2）酶聯(lián)物：          1x12mL，山羊抗兔免疫球蛋白-過氧化物酶共軛物，即用型

3）酰化試劑：        2x3mL，即用型

4）標準品（A-F）：    6x4mL，0；15；50；150；500；2500ng/mL，即用型（ng/mL x 5.67=nmol/L）

5）抗血清：          1x6mL，兔抗血清素抗體，即用型

6）�；�緩沖液：      1x55mL，Tris緩沖液，即用型

7）洗滌緩沖液：      50倍濃縮，1x20mL

8）質(zhì)控品（1/2）：    2x4mL，即用型

9）底物液：          1x12mL，TMB，即用型

10）終止液：         1x12mL，0.25M，即用型

儲存方式

在2-8℃下可儲存至有效期，不要使用過期后的試劑

代測服務(wù)

科潤達生物工程有限公司是DRG有限公司（德爾基）的中國地區(qū)代理，DRG公司由Dr.Geacintov于1970年在美國新澤西州成立，在德國瑪堡市有生產(chǎn)研發(fā)基地，是一家專業(yè)醫(yī)療診斷試劑、設(shè)備的制造商和經(jīng)銷商，并在100多個國家有分支機構(gòu)和合作伙伴。 深圳市科潤達生物工程有限公司作為DRG在中國的代理商，為客戶提供該品牌的進口試劑盒及其售后服務(wù)，科潤達生物公司成立三十多年來一直專攻生物技術(shù)的免疫學領(lǐng)域，與全國各地的高校、疾控、醫(yī)院、檢測機構(gòu)以及科研機構(gòu)保持穩(wěn)定的技術(shù)交流與合作。科潤達生物自有醫(yī)學實驗室，可以為客戶提供“多品種、多方法、多系列”的檢測試劑盒，并且保證快速、及時穩(wěn)定供貨。如果您需要激素試劑盒、立克次體試劑盒、傳染病試劑盒歡迎您與我們聯(lián)系，或請您關(guān)注“科潤達生物”公眾號，科潤達生物提供專業(yè)服務(wù)！

1 INTRODUCTION1.1Intended use and principle of the testEnzyme Immunoassay for the quantitative determination of serotonin in urine and serum, to evaluate serotoninhomeostasis.The determination of serotonin in urine is helpful for the determination of neurostress.The quantitative determination of serotonin follows the basic principles of the enzyme immunoassay.In the first step, serotonin is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plateformat. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples andthe solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium,free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase isdetected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared withknown standard concentrations. Manual processing is recommended. The use of automatic laboratory equipment is theresponsibility of the user.Thisin-vitrodiagnostic is for professional use only.1.2Clinical applicationSerotonin (5-hydroxytryptamine) is an intermediate product of tryptophan metabolism [2], a well-studied neurotransmitter,and may also act as a peripheral hormone [3]. Synthesis occurs mainly in enterochromaffin cells (ec-cells) of thegastrointestinal tract and in neurons [2, 4]. It is present in high concentrations in ec-cells of the intestine, serotonergicneurons of the brain, and platelets [1, 2, 4 – 6]. Serotonin is mainly degraded to 5-hydroxyindole acetic acid (5-HIAA) ormelatonin [2, 7] and can be excreted in the urine [8]. In the bloodstream, the vast majority of serotonin is found in platelets[9] and can be readily detected in serum. Altered serotonin levels in serum and/or urine can indicate both physical andpsychological dysfunction.Serotonin balance may be impaired in serum and/or urine in a variety of conditions. For example, decreased serotoninlevels have been demonstrated in depression, anxiety, and even pain sensitivity compared to unaffected subjects [6, 8,10]. Increased serotonin levels, on the other hand, have been reported in patients with serotonin-secretingneuroendocrine tumors, also called carcinoid tumors [3, 12, 13], or hepatocellular carcinomas [14].Therapeutic consequences should never be based on laboratory results alone even if all test results are in agreementwith the items as under point “Procedural cautions, guidelines and warnings”. Any laboratory result is only a part of thetotal clinical picture of the patient.Only in cases ｗhere the laboratory results are in an acceptable agreement with the overall clinical picture of the patient itcan be used for therapeutic consequences.The test result itself should never be the sole determinant for deriving any therapeutic consequences.

更新時間：2024/10/22 11:02:57