兔抗大鼠/小鼠脂筏蛋白-1抗體
體積：100ug
介紹：筏蛋白富含膠質(zhì)脂蛋白和膽固醇，它被提倡參與蛋白質(zhì)分選的膜結(jié)構(gòu)域。眾所周知，許多信號(hào)轉(zhuǎn)導(dǎo)分子聚集在那里。不像作為另一個(gè)膜的微區(qū)域的Cabeole，區(qū)分捕獲和鑒定過(guò)的脂筏蛋白比較困難，因?yàn)樵摰鞍自诩?xì)胞膜上連續(xù)移動(dòng)，同時(shí)也因?yàn)樵摰鞍踪|(zhì)的大小和結(jié)構(gòu)是不穩(wěn)定的。因此，為了鑒別，需要特定的脂筏蛋白的標(biāo)記。Flotillin作為脂筏蛋白的組成成分，被建議作為脂筏的標(biāo)記物。
抗原：大鼠和小鼠Flotillin-1（SQVNHNKPLRTA）共同位點(diǎn)的C末端部分的合成肽
純化：用抗原肽純化
形式：凍干粉，含有0.05％NaN 3及1％BSA的PBS
使用方法：在該產(chǎn)品中加入1.0ml的去離子水（濃度為：100ug/ml）
穩(wěn)定性：凍干粉，2-8℃可保存5年；溶液狀態(tài)，-20℃可保存2年。
應(yīng)用：該抗體可通過(guò)幾種技術(shù)，例如親和素生物素復(fù)合物（ABC），微波預(yù)處理后的包埋組織（10分鐘，10mM檸檬酸緩沖液，pH值6.0），用于福爾馬林固定石蠟進(jìn)行免疫組化， *優(yōu)濃度約為2μg/ mL，但濃度應(yīng)根據(jù)每個(gè)實(shí)驗(yàn)室進(jìn)行優(yōu)化。
該抗體可用于蛋白質(zhì)印跡實(shí)驗(yàn)，濃度約為2μg/ mL。
特異性：與大鼠，小鼠和人類Flotillin-1反應(yīng)（通過(guò)蛋白質(zhì)印跡和免疫組織化學(xué)證實(shí)）
日本IBL進(jìn)口 科研用抗體，貨源保證，強(qiáng)大的技術(shù)隊(duì)伍。
深圳科潤(rùn)達(dá)生物工程有限公司為廣大客戶提供國(guó)內(nèi)售后技術(shù)支持。
Data Sheet
Code No. 18951
Anti-Mouse/Rat
Flotillin-1 (C) Rabbit IgG Affinity Purify
Introduction : Raft is rich in sphingo-gliycolipids ａnd cholesterol, it is advocated as a membrane domain which participates in protein sorting, ａnd it is known that many signal transduction molecules are gathering there. It is difficult to distinguish catch ａnd identify Raft protein, unlike Cabeole which is another micro domain of membrane, because the protein moves continuously on a cell membrane, ａnd because the size ａnd the structure of the protein are labile. For this reason, the marker specific to Raft was required in order to identify. Flotillin is the composition ingredient of Raft ａnd the possibility as a marker of Raft is suggested.
Antigen
: Synthetic peptide of the C-terminal part of the common site of rat ａnd mouse Flotillin-1 (SQVNHNKPLRTA)
Purification
Form
How to use
Stability
: Purified with antigen peptide
: Lyophilized product from 1 % BSA in PBS containing 0.05 % NaN3
: 1.0 mL deionized water will be added to the product (the conc. comes up 100 μg /mL).
: Lyophilized product, 5 years at 2 - 8 °C
: Solution, 2 years at –20 °C
Application: This antibody can be used for immunohistochemistry with formalin fixed paraffin embedded tissues after microwave pretreatment (10 min, 10mM Citrate Buffer, pH 6.0) by several techniques such as Avidin Biotin Complex (ABC) Method. The optimal concentration is about 2 μg/mL, however, the concentration should be optimized by each laboratory.
: This antibody can be used for western blotting in concentration of about 2 μg /mL.
Specificity: Reacts with rat, mouse ａnd human Flotillin-1 (Confirmed by western blotting and Immunohistochemistry)
更新時(shí)間：2024/10/11 16:13:03
溫馨提示
1.遵守中華人民共和國(guó)有關(guān)法律、法規(guī)，尊重網(wǎng)上道德，承擔(dān)一切因您的行為而直接或間接引起的法律責(zé)任。
2.請(qǐng)您真實(shí)的反映產(chǎn)品的情況,不要捏造、誣蔑、造謠。如對(duì)產(chǎn)品有任何疑問(wèn),也可以留言咨詢。
3.未經(jīng)本站同意，任何人不得利用本留言簿發(fā)布個(gè)人或團(tuán)體的具有廣告性質(zhì)的信息或類似言論。
總訪問(wèn)量：555773 管理登錄
